Artificial Intelligence (AI) and Machine Learning (ML) technologies are transforming the E-Commerce sector by streamlining supply chains, enhancing inventory management, and providing better customer experiences through personalized recommendations. Using real-time data and predictive algorithms, businesses can efficiently manage stock levels and forecast demand. In terms of security, ML and AI provide effective means of detecting and mitigating fraud, protecting personal data, and ensuring secure transactions. This paper delves into the numerous applications and benefits of AI and ML in E-Commerce, highlighting their critical roles in increasing efficiency, improving consumer satisfaction and protecting operations in a fast-paced online market.